abstract：:Current therapies for treatment of hemophilia A involve infusion of factor VIII, but are ineffective for patients who develop inhibitory antibodies. We have previously proposed that bypassing the intrinsic pathway (VIIIa/IXa) with reversibly acylated factor Xa offers an improvement on existing therapies as it provides...

journal_title：Thrombosis research

authors： Lin PH,Laibelman AM,Sinha U

更新日期：1997-11-15 00:00:00

abstract：:We investigated the alteration of heparan sulfate proteoglycans induced by thrombin in cultured vascular endothelial cells. Heparan sulfate proteoglycans, which were metabolically labeled with [3H] glucosamine and [35S] sulfate, were isolated by DEAE-Sephacel ion-exchange chromatography and characterized by molecular ...

journal_title：Thrombosis research

authors： Fujii N,Kaji T,Akai T,Koizumi F

更新日期：1997-11-01 00:00:00

abstract：:Antithrombin (AT) is a serpin capable of trapping thrombin (IIa) in a stable and covalent complex. Complex formation is prevented by leukocyte elastase (LE) cleavage near the AT reactive centre. We mutated the known LE cleavage sites of AT to explore the possibility of producing an LE-resistant AT molecule. Initially,...

journal_title：Thrombosis research

authors： Cunningham MA,Blajchman MA,Sheffield WP

更新日期：1997-10-15 00:00:00

abstract：:We provide a centralised thrombophilia screening service with sample transfer by courier or first class mail, a practice common to many centres. Sample quality is of prime importance, thus we have assessed the effect of delayed sample handling upon the haemostatic variables within our thrombophilia profile. This compr...

journal_title：Thrombosis research

authors： Luddington R,Peters J,Baker P,Baglin T

更新日期：1997-09-15 00:00:00

abstract：:We tested whether inhibition of platelet activation by nitric oxide (NO) might be altered by strenuous exercise. Sixteen healthy male non-smokers, aged 20-26 years, underwent treadmill testing. All subjects reached Bruce stage IV without chest pain or abnormal ST-T wave changes. Platelet aggregation by Born's method a...

journal_title：Thrombosis research

authors： Sakita S,Kishi Y,Numano F

更新日期：1997-09-01 00:00:00

abstract：:The effect of activated protein C (APC) on agonist-induced platelet activation and on thrombin generation after intrinsic (IA) and extrinsic (EA) activation of the coagulation system was studied by flow cytometry and by measuring levels of prothrombin fragment F1+2. In platelet activation studies blood drawn from heal...

journal_title：Thrombosis research

authors： Kaiser B,Jeske W,Hoppensteadt DH,Walenga JM,Drohan W,Fareed J

更新日期：1997-07-15 00:00:00

abstract：:Anticoagulants used for platelet function studies in vitro may affect platelet responsiveness. In the present study we compared the influence of three different anticoagulants, sodium citrate, low molecular mass heparin, and recombinant hirudin, on platelet aggregation in whole blood in vitro, using impedance aggregom...

journal_title：Thrombosis research

authors： Wallén NH,Ladjevardi M,Albert J,Bröijersén A

更新日期：1997-07-01 00:00:00

abstract：:The variant von Willebrand disease (vWd) variant type 2M (Vicenza) was identified in 13 patients of 7 unrelated families. 11 patients were from different parts of germany and 2 patients from Turkey. Hitherto this variant of vWd has been described only in two families originating from the province of Vicenza in Norther...

journal_title：Thrombosis research

authors： Zieger B,Budde U,Jessat U,Zimmermann R,Simon M,Kätzel R,Sutor AH

更新日期：1997-07-01 00:00:00

abstract：:The fibrinolytic system contains a proenzyme plasminogen (Plg) which is converted to plasmin (Plm) by the action of Plg activators. Physiological Plg activators are: tissue-type plasminogen activator (t-PA) and urokinase-type plasminogen activator. Plg was shown to be further cleaved by leukocyte elastase producing se...

journal_title：Thrombosis research

authors： Duboscq C,Genoud V,Parborell MF,Kordich LC

更新日期：1997-06-15 00:00:00

abstract：:Cathepsin A-like enzyme released from human platelets by thrombin hydrolyzed at the highest rate Cbz-Phe-Ala, Cbz-Phe-Met and Cbz-Phe-Leu, did not require activators and was inhibited by DFP, DCI and mercurial compounds (mersalyl acid, PCMS, PCMB and HgCl2). The optimum activity of secreted enzyme was at pH 5.0-6.0. C...

journal_title：Thrombosis research

authors： Ostrowska H

更新日期：1997-06-01 00:00:00

abstract：:The present study was designed to find out whether the synthetic pentasaccharide SR 90107A/Org 31540, which is presently being evaluated in clinical trials as an antithrombotic agent, influences lipoprotein metabolism in rats as determined by plasma triglyceride (TG) lipase activity. A comparison with three clinically...

journal_title：Thrombosis research

authors： Hoffmann P,Bernat A,Dumas A,Petitou M,Hérault JP,Herbert JM

更新日期：1997-05-15 00:00:00

abstract：:Fibrinogen is an important risk factor for atherosclerosis, stroke and cardiovascular heart disease (CHD). This risk is increased when associated with a high serum cholesterol. Furthermore, it is also believed that not only fibrinogen concentration, but also the quality of fibrin networks may be an important risk fact...

journal_title：Thrombosis research

authors： Veldman FJ,Nair CH,Vorster HH,Vermaak WJ,Jerling JC,Oosthuizen W,Venter CS

更新日期：1997-05-01 00:00:00

abstract：:Anticoagulant fucoidan fractions of different molecular weight and sulfate content were prepared and investigated for their effects on platelet function in vitro. The fucoidan fractions were incubated with human platelet rich plasma (PRP) at concentrations of 5, 10 and 50 micrograms/ml. Platelet activation was subsequ...

journal_title：Thrombosis research

authors： Dürig J,Bruhn T,Zurborn KH,Gutensohn K,Bruhn HD,Béress L

更新日期：1997-03-15 00:00:00

abstract：:Since the first description by Dahlbäck et al (1) of a hereditary defect in the plasma of three unrelated thrombophilic patients characterized by a poor anticoagulant response to activated protein C (APC), many reports have shown a high prevalence of the APC resistance in both the general and thrombophilic populations...

journal_title：Thrombosis research

authors： Gouault-Heilmann M,Leroy-Matheron C

更新日期：1997-02-15 00:00:00

abstract：:Some previous reports indicated that a minimal amount of anti-IIa is necessary for the optimal anti-Xa activity of low molecular weight heparin (LMW-H). To know if we could improve the prophylactic activity of LMW-H, providing a mild antithrombin effect, we conducted an experiment in which we administered subcutaneous...

journal_title：Thrombosis research

authors： Pèrez-Requejo JL,Lucena-Solano O,Pérez-García M,Santarelli MT

更新日期：1997-02-01 00:00:00

abstract：:Eight patients with femoro-popliteal or sural DVT, confirmed by phlebography, were treated with intravenous Desmin (LMW-dermatan sulphate): on the first day, after an initial i.v. injection of 400 mg, all patients received an infusion of 800 mg in 500 ml of saline, during 24 hours; this infusion was repeated in each o...

journal_title：Thrombosis research

authors： Kretz JG,Chakfe N,Wiesel ML,Grunebaum L,Zamboni V,Palazzini E,Cazenave JP

更新日期：1996-12-15 00:00:00

abstract：:This paper continues our study (see Part I) where we modeled the spatio-temporal dynamics of the intrinsic pathway of blood coagulation. Here, we analyzed this model and showed that it describes the threshold behavior of coagulation. When activation is subthreshold (which produces not more than 0.07 nM factor XIa at s...

journal_title：Thrombosis research

authors： Zarnitsina VI,Pokhilko AV,Ataullakhanov FI

更新日期：1996-12-01 00:00:00

abstract：:The present investigation has used an aggregation-disaggregation-reaggregation model to determine if exposure to potent aggregating agents causing a refractory state in deaggregated platelets results in down-regulation and clearance of GPIb/IX, the receptor for von willebrand factor. Thrombin, the thrombin receptor ac...

journal_title：Thrombosis research

authors： White JG,Rao GH

更新日期：1996-11-15 00:00:00

abstract：:Pregnancy induces several haemostatic perturbations. Some authors described possible acquired activated protein C resistance (APCR) during normal pregnancy. We wanted to test this possibility and to evaluate if this acquired APCR might contribute to the known increased tendency to thrombosis associated with pregnancy....

journal_title：Thrombosis research

authors： Schlit AF,Col-De Beys C,Moriau M,Lavenne-Pardonge E

更新日期：1996-11-01 00:00:00

abstract：:We characterized the antithrombotic, haemorrhagic, and ex vivo anticoagulant effects of a recently identified depolymerized holothurian glycosaminoglycan (DHG), and compared these effects with those of unfractionated heparin (UFH), low molecular weight heparin (LMWH), and dermatan sulfate (DS). In thrombin-induced ven...

journal_title：Thrombosis research

authors： Kitazato K,Kitazato KT,Nagase H,Minamiguchi K

更新日期：1996-10-15 00:00:00

abstract：:The antithrombotic actions of selective factor Xa (FXa) inhibitors, recombinant tick anticoagulant peptide (rTAP) and DX-9065a, were evaluated in experimental thrombosis models in anesthetized rats. In the first model, thrombosis was induced by exposing flowing blood to a silk thread anchored in an arteriovenous (AV) ...

journal_title：Thrombosis research

authors： Wong PC,Crain EJ Jr,Nguan O,Watson CA,Racanelli A

更新日期：1996-07-15 00:00:00

abstract：:Epsilon-amino-caproic acid (EACA) induces a clinically-useful anti-haemorrhagic, mildly thrombotic state while ellagic acid (EA) induces a severe hypercoagulable one. Reversal of these states may on occasion be necessary. The effectiveness of the antiplatelet drugs, aspirin and dipyridamole (ASA/D), in reducing thromb...

journal_title：Thrombosis research

authors： Iomhair MM,Lavelle SM

更新日期：1996-06-15 00:00:00

abstract：:Aim of this study was to evaluate rapid D-Dimer tests for their utility in diagnosis of acute pulmonary embolism (PE). Tests were performed in 183 consecutive pats referred for lung scanning because of clinically suspected PE. According to lung scans and the clinical course of disease 19 pats were classified to have P...

journal_title：Thrombosis research

authors： Veitl M,Hamwi A,Kurtaran A,Virgolini I,Vukovich T

更新日期：1996-06-01 00:00:00

abstract：:The experiments in this work were focused on determination of the extent to which fibrinogen component of fibrin glue (Hemaseel HMN, Haemacure Biotech.) retained the conformation of the parent molecule after dry heat antiviral treatment (one hour at 100 degrees C). For this purpose antigenic structure of the fibrinoge...

journal_title：Thrombosis research

authors： Cierniewski CS,Pluskota E,Cieslak M,Brodniewicz T,Nowotarski M

更新日期：1996-05-15 00:00:00

abstract：:We have previously reported on a microassay based on human kininogens as cysteine proteinase inhibitors (CPIs), which could quantify partially purified kininogens from different biological fluids (J Pharmacol Meth 26, 113-124, 1991). In the present study we describe a functional method that, when assuming a 1:1 stoich...

journal_title：Thrombosis research

authors： Karlsrud TS,Buø L,Aasen AO,Johansen HT

更新日期：1996-05-01 00:00:00

abstract：:Human fibrinogen exposed to 46.5 degrees C was subjected to gel permeation chromatography. The protein eluted in two distinct peaks. The first peak appeared in the void volume containing soluble fibrinogen aggregates, while the other peak represented monomeric fibrinogen. In contrast to the monomeric peak material, th...

journal_title：Thrombosis research

authors： Bennick A,Haddeland U,Brosstad F

更新日期：1996-04-15 00:00:00

abstract：:In this study, we investigated the effects of a 3-min insulin incubation both at physiological and at supraphysiological concentrations on platelet aggregation and intraplatelet cyclic guanosine monophosphate (cGMP) levels both in the absence and in the presence of phosphodiesterase inhibition. We observed that insuli...

journal_title：Thrombosis research

authors： Anfossi G,Massucco P,Mattiello L,Piretto V,Mularoni E,Cavalot F,Paoletti G,Trovati M

更新日期：1996-04-01 00:00:00

abstract：:Soluble fibrin (SF) has attracted considerable interest as a marker for haemostatic activation. Two new enzyme immunoassays for SF, Enzymun-Test FM (Boehringer Mannheim, Germany) and Fibrinostika Soluble Fibrin (Organon Teknika, Belgium) have recently become commercially available. We measured plasma levels of SF in c...

journal_title：Thrombosis research

authors： Rahr HB,Danielsen D,Andersen BS,Borris LC,Lassen MR

更新日期：1996-03-15 00:00:00

abstract：:Platelet aggregate size was determined with a newly-developed platelet aggregometer, PA-100 (KOWA), which can quantitatively evaluate the size and number of platelet aggregates by means of the particle counting method based upon light scattering. Epinephrine-induced platelet aggregation consists of two phases, the for...

journal_title：Thrombosis research

authors： Satoh K,Ozaki Y,Qi R,Yang L,Asazuma N,Yatomi Y,Kume S

更新日期：1996-03-01 00:00:00

abstract：:To investigate the effects of new drugs on the prevention of venous thrombosis, the Wessler stasis model is extensively employed usually in the rabbit. In this model, a hypercoagulable state is achieved by the administration of either heterologous serum (often human serum) or an activated coagulation factor followed b...

journal_title：Thrombosis research

authors： Millet J,Vaillot M,Theveniaux J,Brown NL

更新日期：1996-02-15 00:00:00

abstract：:Epidemiological studies have shown that oral contraceptives increase the risk of thromboembolic disease in susceptible women however the mechanisms involved are unclear. We investigated whole blood platelet aggregation in 44 women randomly allocated to 6 cycles of treatment with either gestodene (75ug) or desogestrel ...

journal_title：Thrombosis research

authors： Norris LA,Devitt M,Bonnar J

更新日期：1996-02-15 00:00:00

abstract：:We compared the effects of dipyridamole, RA-642, and mopidamol on platelet activity and thromboxane/prostacyclin balance in relation to the degree of retinal vascularization in a model of experimental streptozotocin-induced diabetes in rats. After 3 months, collagen-induced platelet aggregation in whole blood was 25% ...

journal_title：Thrombosis research

authors： De La Cruz JP,Moreno A,Mérida F,García Campos J,Sánchez de la Cuesta F

更新日期：1996-02-01 00:00:00

abstract：:We studied the stability of three markers of coagulation (prothrombin fragment 1 + 2 (F1 + 2), thrombin-antithrombin III complexes (TAT), and soluble fibrin (SF)) in stored frozen plasma, in addition to one marker of fibrinolysis (total fibrinogen degradation products and fibrin degradation products (TDP)). All marker...

journal_title：Thrombosis research

authors： Iversen LH,Thorlacius-Ussing O

更新日期：1996-01-15 00:00:00

abstract：:Use of low molecular weight heparins (LMWHs) is now generally accepted as an ideal method of prophylaxis against postoperative venous thromboembolism. However, the long-term effects on the development of postthrombotic deep vein insufficiency are not known. Every patient developing such complications consumes a vast a...

journal_title：Thrombosis research

authors： Kakkar VV

更新日期：1996-01-01 00:00:00

abstract：:Defibrotide (D) was labelled with 125I or with 32P. The radiolabelled compounds ([125I]-Defibrotide ([125I]-D), [32P]-Defibrotide ([32P]-D) retained the same profibrinolytic activity, in vitro, as the parent drug, suggesting that the labelling procedures had not modified the pharmacological properties of D and hence t...

journal_title：Thrombosis research

authors： Fisher J,Holland TK,Pescador R,Porta R,Ferro L

更新日期：1996-01-01 00:00:00

abstract：:To investigate the relationships between tumor necrosis factor-alpha (TNF), interleukin-1 beta (IL-1 beta), soluble thrombomodulin (TM), and disseminated intravascular coagulation (DIC) in patients with systemic inflammatory response syndrome (SIRS), twenty-nine SIRS patients were classified into three groups; 4 patie...

journal_title：Thrombosis research

authors： Gando S,Kameue T,Nanzaki S,Nakanishi Y

更新日期：1995-12-15 00:00:00

abstract：:The effect of dalteparin, a low molecular weight heparin, on severely antithrombin III (ATIII)-decreased disseminated intravascular coagulation (DIC) model was compared with that of unfractionated heparin (heparin). The DIC model in rabbits was produced by continuous infusion of thrombin in combination with bolus inje...

journal_title：Thrombosis research

authors： Tazawa S,Ichikawa K,Misawa K,Fukuyama J,Hamano S,Miyata H,Sakuragawa N

更新日期：1995-12-01 00:00:00

abstract：:Defibrotide both "in vitro" and "ex vivo" decreases PMN adhesion to nylon columns. This activity is blunted by 8-phenyltheophylline, a known blocker of adenosine A1/A2 receptors, suggesting that Defibrotide acts on that kind of receptors. Indomethacin, a blocker of PGI2 synthesis, did not cancel DFT's activity, sugges...

journal_title：Thrombosis research

authors： Alberico P,Porta R,Pescador R,Ferro L

更新日期：1995-11-15 00:00:00

abstract：:The pharmacodynamic and pharmacokinetic properties of endpoint-attached N'alkylamine derivatives of low molecular mass heparin (LMMH), low molecular mass heparin (LMMH), low molecular mass heparin-tyramine (LMMH-tyr) and low molecular mass heparin-tyramine-fluorescein-5-isothiocyanate (LMMH-tyr-fitc) were investigated...

journal_title：Thrombosis research

authors： Malsch R,Piazolo L,Harenberg J,Heene DL

更新日期：1995-11-01 00:00:00

abstract：:The effects of platelets on clot lysis and plasminogen activation by staphylokinase (SAK) were investigated. At concentrations ranging from 2 x 10(-7) to 1 x 10(-5)g/ml of SAK, the lysis time of platelet-rich plasma clots (PRP-clots) was shorter than that of platelet-poor plasma clots (PPP-clots). This reduction of cl...

journal_title：Thrombosis research

authors： Suehiro A,Tsujioka H,Yoshimoto H,Ueda M,Higasa S,Kakishita E

更新日期：1995-10-15 00:00:00